Human MMP-2 Antibody

Name: Human MMP-2 Antibody
Brand: R&D Systems
SKU: MAB902

R&D Systems, part of Bio-Techne | Catalog # MAB902

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Dual RNAscope ISH-IHC Compatible, Immunohistochemistry, Western Blot

Cited:

Cell-based ELISA, ELISA Development, Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 36006

View all MMP-2 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-2

Specificity

Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human MMP-2 Antibody

MMP-2 in Human Ovarian Cancer Tissue.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-2 in Human Ovarian Cancer Tissue.

MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of MMP-2 in Human Stomach.

Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for MMP2 mRNA (ACD RNAScope Probe, catalog #311751; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human MMP2 monoclonal antibody (R&D Systems catalog # MAB902) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts.

Applications for Human MMP-2 Antibody

Application

Recommended Usage

Dual RNAscope ISH-IHC Compatible

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Immunohistochemistry

50-100 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025) under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

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Product Specific Notices for Human MMP-2 Antibody

For research use only

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