Human/Mouse FoxO3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6165
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxO3
Met372-Gly673
Accession # O43524
Met372-Gly673
Accession # O43524
Specificity
Detects human and mouse FoxO3 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse FoxO3 Antibody
Detection of Human FoxO3 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6165) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxO3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FoxO3 in MCF‑7 Human Cell Line.
FoxO3 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human FoxO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6165) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human FoxO3 by Simple WesternTM.
Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO3 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse FoxO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6165) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse FoxO3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Knockout Validated
FoxO3
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
FoxO3 knockout HEK293T cell line.
Simple Western
10 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and PC‑3 human prostate cancer cell line
Sample: Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and PC‑3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxO3
Forkhead box O3 (FoxO3) is a ubiquitously expressed 72 kDa transcriptional regulator that is involved in cellular differentiation, angiogenesis, tumor progression, apoptosis, and the responses to oxidative stress and DNA damage. Phosphorylation of FoxO3 by Akt induces its association with 14-3-3 proteins and its retention in the cytoplasm. In response to the loss of survival factors, dephosphorylation of FoxO3 induces its translocation to the nucleus where it promotes apoptosis. Its level of acetylation is regulated in response to cellular metabolic requirements. Within amino acids 372‑673 (C-terminal to the DNA binding domain), human FoxO3 shares 95% aa sequence identity with mouse and rat FoxO3.
Long Name
Forkhead Box O3
Alternate Names
AF6q21, FKHRL1, FKHRL1P2, FOXO3A
Gene Symbol
FOXO3
UniProt
Additional FoxO3 Products
Product Documents for Human/Mouse FoxO3 Antibody
Product Specific Notices for Human/Mouse FoxO3 Antibody
For research use only
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