Human/Mouse GLI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3455
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806
Met1-Glu237
Accession # P47806
Specificity
Detects human and mouse GLI-1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse GLI-1 Antibody
Detection of Human GLI‑1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GLI‑1 in HeLa Human Cell Line.
GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse GLI-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human GLI-1
Sample: HEK293 human embryonic kidney cell line transfected with human GLI-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using AF3455 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-1
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
UniProt
Additional GLI-1 Products
Product Documents for Human/Mouse GLI-1 Antibody
Product Specific Notices for Human/Mouse GLI-1 Antibody
For research use only
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