Human/Mouse GLI-1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3455
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806
Met1-Glu237
Accession # P47806
Specificity
Detects human and mouse GLI-1 in Western blot. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) GLI-2 and rmGLI-3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse GLI-1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human GLI-1
Recombinant Mouse GLI-1
Sample: Recombinant Human GLI-1
Recombinant Mouse GLI-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-1
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
UniProt
Additional GLI-1 Products
Product Documents for Human/Mouse GLI-1 Biotinylated Antibody
Product Specific Notices for Human/Mouse GLI-1 Biotinylated Antibody
For research use only
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