Human/Mouse GSK-3 alpha/ beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB25063
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 833916
Product Specifications
Immunogen
E. coli-derived recombinant human GSK-3 beta
Asp200-Phe305
Accession # P49841
Asp200-Phe305
Accession # P49841
Specificity
Detects human GSK-3 beta in direct ELISAs and human GSK-3 alpha and GSK-3 beta in Western blots. Detects human and mouse GSK-3 alpha/ beta in immunocytochemistry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse GSK-3 alpha/ beta Antibody
Detection of Human GSK 3 alpha and GSK‑3 beta by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GSK-3a at approximately 46 and GSK-3 beta at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GSK‑3 alpha/ beta in HT‑29 Human Cell Line.
GSK-3a/ beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GSK‑3 alpha/ beta in NIH‑3T3 Mouse Cell Line.
GSK-3a/ beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse GSK-3 alpha/ beta Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
2 µg/mL
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GSK-3 alpha/beta
Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.
Long Name
Glycogen Synthase Kinase 3
Alternate Names
DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha
UniProt
Additional GSK-3 alpha/beta Products
Product Documents for Human/Mouse GSK-3 alpha/ beta Antibody
Product Specific Notices for Human/Mouse GSK-3 alpha/ beta Antibody
For research use only
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