Human/Mouse MEF2C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6786
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 681824
Product Specifications
Immunogen
E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413
Ala135-Lys239
Accession # Q06413
Specificity
Detects human MEF2C in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human SRF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse MEF2C Antibody
MEF2C in C2C12 Mouse Cell Line.
MEF2C was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nucleus of myoblasts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MEF2C in Human Brain.
MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse MEF2C Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using MAB6786 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEF2C
Long Name
Myocyte-specific Enhancer Factor 2C
Alternate Names
DEL5q14.3
Gene Symbol
MEF2C
UniProt
Additional MEF2C Products
Product Documents for Human/Mouse MEF2C Antibody
Product Specific Notices for Human/Mouse MEF2C Antibody
For research use only
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