Human/Mouse PDX-1/IPF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2419
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 267712
Product Specifications
Immunogen
E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945
Ala91-Arg283
Accession # P52945
Specificity
Detects human and mouse PDX-1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse PDX-1/IPF1 Antibody
Detection of Mouse PDX‑1/IPF1 by Western Blot.
Western blot shows lysates of betaTC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDX‑1/IPF1 in betaTC‑6 Mouse Cell Line.
PDX-1/IPF1 was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells.
PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse PDX-1/IPF1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed betaTC-6 mouse beta cell insulinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Sample: Immersion fixed betaTC-6 mouse beta cell insulinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: betaTC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Sample: betaTC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Western Blot
1 µg/mL
Sample: betaTC-6 mouse beta cell insulinoma cell line
Sample: betaTC-6 mouse beta cell insulinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB2419 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
Long Name
Pancreas/Duodenum Homeobox-1
Alternate Names
IDX1, IPF1
Gene Symbol
PDX1
UniProt
Additional PDX-1/IPF1 Products
Product Documents for Human/Mouse PDX-1/IPF1 Antibody
Product Specific Notices for Human/Mouse PDX-1/IPF1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...