Human/Mouse/Rat alpha Tubulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9344
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Primate - Cercopithecus aethiops (African Green Monkey)
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Westen Blot, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 961216
Product Specifications
Immunogen
Human TUBA1A synthetic peptide
Accession # Q71U36
Accession # Q71U36
Specificity
Detects human alpha Tubulin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat alpha Tubulin Antibody
Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha Tubulin in SH‑SY5Y Human Cell Line.
a Tubulin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
alpha Tubulin in Human Brain.
a Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat alpha Tubulin Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Western Blot
0.05 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB9344 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha Tubulin
Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth). This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1
Gene Symbol
TUBA1A
UniProt
Additional alpha Tubulin Products
Product Documents for Human/Mouse/Rat alpha Tubulin Antibody
Product Specific Notices for Human/Mouse/Rat alpha Tubulin Antibody
For research use only
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