Human/Mouse/Rat/Chicken Pax7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1675
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Cited:
Human, Mouse, Rat, Porcine, Avian - Chicken, Avian - Duck, Fish - Danio rerio (Zebrafish), Frog - Xenopus (African Clawed Frog), Ovine
Applications
Validated:
Immunocytochemistry, Immunohistochemistry
Cited:
Flow Cytometry, IHC frozen fixed, IHC/IF, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PAX7
Product Specifications
Immunogen
E. coli-derived recombinant chicken Pax7
aa 352-523
aa 352-523
Specificity
Detects human, mouse, rat, and chicken Pax7 (3-8).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat/Chicken Pax7 Antibody
Pax7 in C2C12 Mouse Cell Line.
Pax7 was detected in immersion fixed C2C12 mouse myoblast cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Pax7 in iBJ6 iPS Cell Line.
Pax7 was detected in immersion fixed iBJ6 iPS cell line differentiated into myoblasts using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Pax7 in Human Skeletal Muscle.
Pax7 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat/Chicken Pax7 Monoclonal Antibody (Catalog # MAB1675) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat/Chicken Pax7 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line and iBJ6 iPS cell line differentiated into myoblasts
Sample: Immersion fixed C2C12 mouse myoblast cell line and iBJ6 iPS cell line differentiated into myoblasts
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Reviewed Applications
Read 4 reviews rated 4.8 using MAB1675 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax7
References
- Jostes, B. et al. (1990) Mech. Dev. 33(1):27.
- Asakura, A. et al. (2001) Differentiation 68(4-5):245.
- Kawakami, A. et al. (1997) Mech. Dev. 66:119.
- Ericson, J. et al. (1996) Cell 87:661.
- Berggren, K. et al. (2001) Dev. Dyn. 222:1.
- Takebayashi, H. et al. (2002) Mech. Dev. 113:169.
- McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.
- Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.
Long Name
Paired Box Gene 7
Alternate Names
HUP1, RMS2
Gene Symbol
PAX7
Additional Pax7 Products
Product Documents for Human/Mouse/Rat/Chicken Pax7 Antibody
Product Specific Notices for Human/Mouse/Rat/Chicken Pax7 Antibody
For research use only
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