Human/Mouse/Rat GRK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4339
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRK2
Pro468-Leu689
Accession # P25098
Pro468-Leu689
Accession # P25098
Specificity
Detects endogenous human, mouse, and rat GRK2.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GRK2 Antibody
Detection of Human/Mouse/Rat GRK2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4339) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK2 at approximately 79 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GRK2 in Human Alzheimer's Disease Brain.
GRK2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain using Human/Mouse/Rat GRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4339) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human GRK2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and GRK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat GRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4339) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK2 at approximately 76 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat GRK2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Knockout Validated
GRK2
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
GRK2 knockout HEK293T cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRK2
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the betaARK subfamily, and was originally termed beta-adrenergic receptor kinase 1 ( betaARK1).
Long Name
G protein-coupled Receptor Kinase 2
Alternate Names
ADRBK1, BARK1, Beta-ARK1
Gene Symbol
GRK2
UniProt
Additional GRK2 Products
Product Documents for Human/Mouse/Rat GRK2 Antibody
Product Specific Notices for Human/Mouse/Rat GRK2 Antibody
For research use only
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