Human/Mouse/Rat NF-L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB22163
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1002615
Product Specifications
Immunogen
68kD purified NF-L from Bovine spinal cord.
Specificity
Detects human NF-L in direct ELISAs. Detects human, mouse, and rat NF-L in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat NF-L Antibody
Detection of Human, Mouse, and Rat NF‑L by Western Blot.
Western blot shows lysates of human motor cortex tissue, mouse hypothalamus tissue, and rat hypothalamus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NF-L Monoclonal Antibody (Catalog # MAB22163) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NF-L at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NF‑L in Human Brain.
NF-L was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat NF-L Monoclonal Antibody (Catalog # MAB22163) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat NF-L Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Western Blot
2 µg/mL
Sample: Human motor cortex tissue, Mouse hypothalamus tissue, and Rat hypothalamus tissue
Sample: Human motor cortex tissue, Mouse hypothalamus tissue, and Rat hypothalamus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-L
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Entrez Gene IDs
4747 (Human)
Gene Symbol
NEFL
Additional NF-L Products
Product Documents for Human/Mouse/Rat NF-L Antibody
Product Specific Notices for Human/Mouse/Rat NF-L Antibody
For research use only
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