Human/Mouse/Rat NF-L Antibody

Detection of Human, Mouse, and Rat NF‑L by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB22161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF-L at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

NF‑L in Human Brain.

NF-L was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB22161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of NEFL mRNA (red) and protein (green/blue) in Human Cerebellum.

NEFL mRNA (red) and protein (green/blue) was detected in formalin-fixed paraffin-embedded tissue sections of human cerebellum. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-NEFL (Catalog # 468671) and mouse anti-human NEFL monoclonal antibody (R&D Systems Catalog #MAB22161) at 5 µg/mL. Tissue was stained using RNAscope® 2.5 HD Detection Reagents-RED (Catalog # 322360), Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and and RNAscope® 2.5 LS Green Accessory Pack (Catalog #322550). Tissue was counterstained with 50% hematoxylin (purple).