Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4465

R&D Systems, part of Bio-Techne
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AF4465
AF4465-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all PKC iota/lambda/zeta Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC iota
Ile455-Val596
Accession # P41743

Specificity

Detects human, mouse and rat PKC iota/ lambda/ zeta in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

Detection of Human, Mouse, and Rat PKC?/?/? antibody by Western Blot.

Detection of Human, Mouse, and Rat PKC iota/ lambda/ zeta by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human PKC iota, PKC zeta, and PKC alpha (2 ng/lane) were included. Specific bands were detected at approximately 22 kDa for recombinant PKC iota/zeta and ~80 kDa for natural PKC iota/zeta (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKC?/?/? antibody in Human Pancreas by Immunohistochemistry (IHC-P).

PKC iota/ lambda/ zeta in Human Pancreas.

PKC iota/lambda/zeta was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human/Mouse/Rat PKC iota/lambda/zeta Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.

Detection of Human PKC iota/ lambda/ zeta by Simple WesternTM.

Simple Western shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC iota/ lambda/ zeta at approximately 80 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKC iota/ lambda/ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4465). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF4465 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC iota/lambda/zeta

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their co‑factor requirements: conventional PKC isoforms ( alpha, betaΙ, betaΙΙ, and gamma) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms ( delta, epsilon, eta, and theta), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota, lambda, and zeta), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota has 72% overall identity to PKC zeta. Atypical PKCs have been shown to serve as a convergent downstream target for the PI 3-kinase and TC10 signaling pathways. Stimulation of atypical PKCs by TNF-alpha has been shown to be required for
NF‑ kappaB activation. Furthermore, insulin-stimulated atypical PKC activation has been directly implicated in the translocation of GLUT4 and glucose uptake in adipocytes.

Long Name

Protein Kinase C iota/lambda/zeta

UniProt

P41743

Additional PKC iota/lambda/zeta Products

Product Documents for Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PKC iota/ lambda/ zeta Antibody

For research use only

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