Human/Mouse/Rat UCH-L1/PGP9.5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB60072

R&D Systems, part of Bio-Techne
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MAB60072-100
MAB60072-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 972119

View all UCH-L1/PGP9.5 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCH-L1/PGP9.5
Gln2-Ala223
Accession # P09936

Specificity

Detects human UCH-L1/PGP9.5 in direct ELISAs. Detects human, mouse, and rat in Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat UCH-L1/PGP9.5 Antibody

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 antibody by Western Blot.

Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for UCH-L1/PGP9.5 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UCH-L1/PGP9 by Immunocytochemistry (ICC).5 antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

UCH-L1/PGP9.5 in A172 Human Cell Line.

UCH-L1/PGP9.5 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
UCH-L1/PGP9 by Immunohistochemistry (IHC-P).5 antibody in Human Brain by Immunohistochemistry (IHC-P).

UCH-L1/PGP9.5 in Human Brain.

UCH-L1/PGP9.5 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human UCH-L1/PGP9.5 Monoclonal Antibody (Catalog # MAB60072) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Applications for Human/Mouse/Rat UCH-L1/PGP9.5 Antibody

Application
Recommended Usage

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)

Simple Western

1 µg/mL
Sample: A172 human glioblastoma cell line and Neuro‑2A mouse neuroblastoma cell line

Western Blot

0.1 µg/mL
Sample: A172 human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L1/PGP9.5

UCH-L1 (ubiquitin carboxyterminal hydrolase isozyme 1; also PGP9.5) is a 24-27 kDa member of the peptidase C12 family of enzymes. It shows restricted expression, being found in neurons and oocytes. UCH-L1 has dual enzymatic activity. As a monomer, it is a ubiquitin hydrolase that removes ubiquitin from modified proteins; as a homodimer, it acts as a ligase that creates ubiquitin dimers. In neurons, UCH-L1’s most important role appears to be that of generating free ubiquitin. Human UCH-L1 is 223 amino acids (aa) in length. It is O-glycosylated, ubiquitinated, and farnesylated; when farnesylated, it becomes associated with cell membranes. Three potential splice forms are reported. One shows a two aa substitution for aa 12-15, a second contains an alternative start site at Met82, and a third shows the same start site coupled with a deletion of aa 138-153. Full-length human UCH-L1 shares 95% aa identity with mouse UCH-L1.       

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 22223 (Mouse)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for Human/Mouse/Rat UCH-L1/PGP9.5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat UCH-L1/PGP9.5 Antibody

For research use only

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