Human/Mouse STAT1 p91 Antibody

Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human STAT1 by Simple Western^TM.

Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for STAT1 at approximately 84 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.