Human Neutrophil Elastase/ELA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB91671R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 950317R
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neutrophil Elastase/ELA2
Met1-Asn252
Accession # P08246
Met1-Asn252
Accession # P08246
Specificity
Detects human Neutrophil Elastase/ELA2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Neutrophil Elastase/ELA2 Antibody
Detection of Human Neutrophil Elastase/ELA2 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Neutrophil Elastase/ELA2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Neutrophil Elastase/ELA2 in THP‑1 Human Cell Line.
Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Neutrophil Elastase/ELA2 in Human Tonsil.
Neutrophil Elastase/ELA2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB91671R) at 0.05 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neutrophils. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Neutrophil Elastase/ELA2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Immunohistochemistry
0.05-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
1 µg/mL
Sample: Human Lung Tissue
Sample: Human Lung Tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB91671R in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neutrophil Elastase/ELA2
Trappin-2/Elafin (7-11). Its activity in the lung is increased by exposure to tobacco smoke which inactivates Serpin A1 through methionine oxidation (12). Mature human Neutrophil Elastase shares 73% amino acid sequence identity with mouse and rat Neutrophil Elastase (13, 14). Multiple mutations in the human ELANE gene are causative of severe congenital and cyclic neutropenias (15).
References
- Korkmaz, B. et al. (2010) Pharmacol. Rev. 62:726.
- Stein, R.L. et al. (1987) Biochemistry 26:1301.
- Bachovchin, W.W. (1986) Biochemistry 25:7751.
- Garwicz, D. et al. (2005) Haematologica 90:38.
- Owen, C.A. et al. (1995) J. Cell Biol. 131:775.
- Stephan, A. and M. Fabri (2015) Exp. Dermatol. 24:161.
- Carrell, R.W. et al. (1982) Nature 298:329.
- Rice, W.G. and S.J. Weiss (1990) Science 249:178.
- Thompson, R.C. et al. (1986) Proc. Natl. Acad. Sci. USA 83:6692.
- Cooley, J. et al. (2001) Biochemistry 40:15762.
- Wiedow, O. et al. (1990) J. Biol. Chem. 265:14791.
- Taggart, C. et al. (2000) J. Biol. Chem. 275:27258.
- Sinha, S. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2228.
- Okano, K. et al. (1987) J. Biochem. 102:13.
- Makaryan, V. et al. (2015) Curr. Opin. Hematol. 22:3.
Alternate Names
ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin
Gene Symbol
ELANE
UniProt
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Human Neutrophil Elastase/ELA2 Antibody
Product Specific Notices for Human Neutrophil Elastase/ELA2 Antibody
For research use only
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