Human NF-H Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB31083
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1053309
Product Specifications
Immunogen
Natural Neurofilament H (200 kD) Bovine Protein
Specificity
Detects Human and Bovine NF-H in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human NF-H Antibody
Detection of NF-H in Human Brain Cerebellum.
NF-H was detected in immersion fixed paraffin-embedded sections of human brain cerebellum using Mouse Anti-Human NF-H Monoclonal Antibody (Catalog # MAB31083) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinjie neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of NF-H in HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells.
NF-H was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells using Mouse Anti-Human NF-H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of NF-H in K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells.
NF-H was detected in immersion fixed K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells using Mouse Anti-Human NF-H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human NF-H Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa (positive), K562 (negative) cells and Raji (negative) cells.
Sample: Immersion fixed HeLa (positive), K562 (negative) cells and Raji (negative) cells.
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cerebellum
Sample: Immersion fixed paraffin-embedded sections of human brain cerebellum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-H
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Entrez Gene IDs
4744 (Human)
Gene Symbol
NEFH
Additional NF-H Products
Product Documents for Human NF-H Antibody
Product Specific Notices for Human NF-H Antibody
For research use only
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