Human Perilipin-2 Antibody

Detection of Human Perilipin-2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Perilipin-2 in HepG2 Human Cell Line.

Perilipin-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Perilipin-2 in Human Liver.

Perilipin-2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.