Human Phospho-IkB-alpha (S32/S36) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4809
Discontinued Product
AF4809 has been discontinued.
View all IkB-alpha products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Multiplex Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human IkB-alpha S32/36 sites
Specificity
Detects human IkB-alpha when phosphorylated at S32/36.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-IkB-alpha (S32/S36) Antibody
Detection of Human Phospho-IkB‑ alpha (S32/S36) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-a (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-a (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-a Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-IkB-alpha (S32/S36) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-alpha
NF kappaB inhibitor alpha (IkB‑ alpha) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues 32 and 36 marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Gene Symbol
NFKBIA
Additional IkB-alpha Products
Product Documents for Human Phospho-IkB-alpha (S32/S36) Antibody
Product Specific Notices for Human Phospho-IkB-alpha (S32/S36) Antibody
For research use only
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