Human IkB-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4299
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 417208
Product Specifications
Immunogen
E. coli-derived recombinant human IkB-alpha
Met1-Leu317
Accession # P25963
Met1-Leu317
Accession # P25963
Specificity
Detects human IkB-alpha in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IkB-alpha Antibody
Detection of Human IkB-alpha by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-a at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
IkB‑ alpha in Human Prostate Cancer Tissue.
IkB-a was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human IkB‑ alpha by Simple WesternTM.
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for IkB‑ alpha at approximately 44 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human IkB-alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Knockout Validated
IkB‑ alpha
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
IkB‑ alpha knockout HEK293T cell line.
Simple Western
1 µg/mL
Sample: LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line
Sample: LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line
Western Blot
0.1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line
Sample: Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-alpha
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Gene Symbol
NFKBIA
UniProt
Additional IkB-alpha Products
Product Documents for Human IkB-alpha Antibody
Product Specific Notices for Human IkB-alpha Antibody
For research use only
Loading...
Loading...
Loading...
Loading...