Human PILR-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4189
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR-beta isoform 1
Gln20-Ala189
Accession # Q9UKJ0
Gln20-Ala189
Accession # Q9UKJ0
Specificity
Detects recombinant human PILR-beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PILR-beta Antibody
Detection of PILR‑ beta in Human Peripheral Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4189, filled histogram) or control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (NL010).Applications for Human PILR-beta Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human PILR-beta
Sample: Recombinant Human PILR-beta
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PILR-beta
Paired immunoglobulin-like, type 2 receptor beta (PILR-beta) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-alpha inhibitory receptor. PILR-beta is expressed in a wide variety of tissues including hematopoietic cells. Mature human PILR-beta is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM‑bearing adaptor molecules. Within the V-type Ig-like region in their ECD, human PILR-beta and PILR-alpha share a 92% aa sequence identity. The aa sequence of mouse PILR-beta ECD is only 43% identical to that of the human protein.
Long Name
Paired-Ig-likeType 2 Receptor beta
Alternate Names
FDFACT1, FDFACT2, PILRB, PILRbeta
Gene Symbol
PILRB
UniProt
Additional PILR-beta Products
Product Documents for Human PILR-beta Antibody
Product Specific Notices for Human PILR-beta Antibody
For research use only
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