Human PLTP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5109
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1
Glu18-Val493 (Glu18Val)
Accession # P55058
Glu18-Val493 (Glu18Val)
Accession # P55058
Specificity
Detects human PLTP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PLTP is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PLTP Antibody
Detection of human PLTP by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human PLTP Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PLTP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLTP Antibody
Application
Recommended Usage
Knockout Validated
PLTP
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
PLTP knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLTP
Long Name
Phospholipid Transfer Protein
Alternate Names
HDLCQ9, Lipid transfer protein II, phospholipid transfer protein
Gene Symbol
PLTP
UniProt
Additional PLTP Products
Product Documents for Human PLTP Antibody
Product Specific Notices for Human PLTP Antibody
For research use only
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