Human Presenilin-1 C-Terminal Fragment Antibody
R&D Systems, part of Bio-Techne | Catalog # AF166
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Presenilin-1
aa 298-407
aa 298-407
Specificity
Detects human Presenilin‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Presenilin‑1 N-Terminal Fragment (aa 1‑80), Presenilin‑2 C‑Terminal Fragment (aa 322‑388), and rhPresenilin‑2N‑Terminal Fragment (aa 1‑80) is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Presenilin-1 C-Terminal Fragment Antibody
Detection of Human Presenilin‑1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Presenilin‑1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Presenilin-1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Presenilin-1 C-Terminal Fragment Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Knockout Validated
Presenilin‑1
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Presenilin‑1 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Presenilin-1
Additional Presenilin-1 Products
Product Documents for Human Presenilin-1 C-Terminal Fragment Antibody
Product Specific Notices for Human Presenilin-1 C-Terminal Fragment Antibody
For research use only
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