Human/Primate IL-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB602
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human, Mouse, Primate - Papio anubis (Olive Baboon)
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunocytochemistry
Cited:
ELISA Development, ELISA Development (Capture), In vivo assay, Luminex Development, Neutralization, Stimulation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 5355
Product Specifications
Immunogen
E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # NP_000577
Ala21-Thr153
Accession # NP_000577
Specificity
Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-3, rhIL-4, rhIL‑6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Primate IL-2 Antibody
IL-2 in Human PBMCs.
IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL-2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Primate IL-2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin
Human/Primate IL-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB602 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2
Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Human/Primate IL-2 Antibody
Product Specific Notices for Human/Primate IL-2 Antibody
For research use only
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