Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61093) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Detection of ROR gammat/RORC2/NR1F3 in HepG2 cells by Flow Cytometry.

HepG2 cells were stained with Rabbit Anti-Human ROR gammat/RORC2/NR1F3 Monoclonal Antibody (Catalog # MAB61093, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human ROR gammat/RORC2/NR1F3 Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012) and HepG2 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gammat, has been shown to be specifically expressed in the thymus.

Long Name

Retinoic Acid-Related Orphan Receptor gamma t

Alternate Names

RORC2

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449

Additional ROR gamma t/RORC2/NR1F3 Products

Product Documents for Human ROR gammat/RORC2/NR1F3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ROR gammat/RORC2/NR1F3 Antibody

For research use only

Citations
Reviews

Protocols
FAQs

Calculators
Flow Cytometry

Human ROR gammat/RORC2/NR1F3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB61093

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ROR gamma t/RORC2/NR1F3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ROR gammat/RORC2/NR1F3 Antibody

Detection of ROR gamma/RORC/NR1F3 in Human PBMCs by Flow Cytometry.