Human SMAC/Diablo Antibody
R&D Systems, part of Bio-Techne | Catalog # AF789
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SMAC/Diablo
Ala56-Asp239
Accession # Q9NR28
Ala56-Asp239
Accession # Q9NR28
Specificity
Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 μg/mL anti-SMAC/Diablo concentration is used.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human SMAC/Diablo Antibody
Detection of Human SMAC/Diablo by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Detection of Human SMAC/Diablo by Simple WesternTM.
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SMAC/Diablo Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line
Sample: SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line
Western Blot
0.5 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 5 using AF789 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMAC/Diablo
SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.
References
- Du, C. et al. (2000) Cell 102:33.
- Chai, J. et al. (2000) Nature 406:855.
- Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
- Ekert, P. et al. (2001) J. Cell Biol. 152:483.
- Verhagen, A. (2000) Cell 102:43.
Long Name
Second Mitochondria-derived Activator of Caspase
Alternate Names
Diablo
Entrez Gene IDs
56616 (Human)
Gene Symbol
DIABLO
UniProt
Additional SMAC/Diablo Products
Product Documents for Human SMAC/Diablo Antibody
Product Specific Notices for Human SMAC/Diablo Antibody
For research use only
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