Human ST8 alpha-2,8‑Sialyltransferase 8B/
ST8SIA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6590
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human alpha-2,8‑Sialyltransferase 8B/ST8SIA2
Asp24-Thr375
Accession # Q92186
Asp24-Thr375
Accession # Q92186
Specificity
Detects human alpha-2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ST8 alpha-2,8‑Sialyltransferase 8B/ST8SIA2 Antibody
Detection of Human alpha-2,8‑Sialyltransferase 8B/ST8SIA2 by Western Blot.
Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, Saos-2 human osteosarcoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha-2,8‑Sialyltransferase 8B/ ST8SIA2 in Mouse Embryo.
a-2,8-Sialyltransferase 8B/ ST8SIA2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of alpha‑2,8‑Sialyltransferase 8B/ST8SIA2 in IMR‑32 Human Cell Line by Flow Cytometry.
IMR-32 human neuroblastoma cell line was stained with Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human ST8 alpha-2,8‑Sialyltransferase 8B/ST8SIA2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: IMR‑32 human neuroblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: IMR‑32 human neuroblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.2 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line, Saos‑2 human osteosarcoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Sample: 786‑O human renal cell adenocarcinoma cell line, Saos‑2 human osteosarcoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2
References
- Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
- Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
- Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
- Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
- Ong, E. et al. (1998). Glycobiology 8:415.
- Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
- Thompson, M. G. et al. (2010) J. Biol. Chem. in press.
- Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.
Long Name
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
Alternate Names
SIAT8B, ST8SiaII, STX
Entrez Gene IDs
8128 (Human)
Gene Symbol
ST8SIA2
UniProt
Additional ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Products
Product Documents for Human ST8 alpha-2,8‑Sialyltransferase 8B/ST8SIA2 Antibody
Product Specific Notices for Human ST8 alpha-2,8‑Sialyltransferase 8B/ST8SIA2 Antibody
For research use only
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