Human TCEB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11569
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1076415
Product Specifications
Immunogen
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex
Accession # Q15369
Accession # Q15369
Specificity
Detects hElongin B/Elongin C/VHL/CUL2/RBX1 Complex in Direct ELISA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TCEB1 Antibody
Detection of TCEB1 in Human Liver.
TCEB1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of TCEB1 in MCF-7 Human Cell Line.
TCEB1 was detected in fixed MCF-7 human breast cancer cell line using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TCEB1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: fixed MCF-7 human breast cancer cell line
Sample: fixed MCF-7 human breast cancer cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCEB1
References
- Brugarolas J. Molecular genetics of clear-cell renal cell carcinoma. J Clin Oncol. 2014 Jun 20;32(18):1968-76. doi: 10.1200/JCO.2012.45.2003. Epub 2014 May 12. PMID: 24821879; PMCID: PMC4050206.
- Stebbins CE, Kaelin WG Jr, Pavletich NP. Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function. Science. 1999 Apr 16;284(5413):455-61. doi: 10.1126/science.284.5413.455. PMID: 10205047.
- 3.Hakimi AA, Tickoo SK, Jacobsen A, Sarungbam J, Sfakianos JP, Sato Y, Morikawa T, Kume H, Fukayama M, Homma Y, Chen YB, Sankin A, Mano R, Coleman JA, Russo P, Ogawa S, Sander C, Hsieh JJ, Reuter VE. TCEB1-mutated renal cell carcinoma: a distinct genomic and morphological subtype. Mod Pathol. 2015 Jun;28(6):845-853. doi: 10.1038/modpathol.2015.6. Epub 2015 Feb 13. PMID: 25676555; PMCID: PMC4449825.
Long Name
TCEB1
Alternate Names
ELOC, elongin 15 kDa subunit, elongin-C, RNA polymerase II transcription factor SIII subuni, SIII, SIII p15, transcription elongation factor B (SIII), polypept, transcription elongation factor B polypeptide 1, transcription elongation factor B subunit 1
Gene Symbol
Q15369
UniProt
Additional TCEB1 Products
Product Documents for Human TCEB1 Antibody
Product Specific Notices for Human TCEB1 Antibody
For research use only
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