Human Tryptase alpha/beta-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37961
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 349414
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha/beta-1
Ile31-Pro275
Accession # AAD13876
Ile31-Pro275
Accession # AAD13876
Specificity
Detects human Tryptase alpha/beta-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TPS1, rhTPSG1, recombinant mouse (rm) TPS5, rmMCPT1, rmMCPT6, rmMCPT7, or rmMCPT11 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Tryptase alpha/beta-1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE).
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE).
Western Blot
1 µg/mL
Sample: Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)
Sample: Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase alpha/beta-1
Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). They are stored in secretory granules of mast cells, where they form active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, beta tryptases are resistant to macromolecule protease inhibitors (2). When mast cells are activated, beta tryptases are released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). beta tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.
References
- Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
- Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
- Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.
Alternate Names
TPS2, TPSB2, Tryptase beta-2, Tryptase beta2, Tryptase-C
Gene Symbol
TPSB2
UniProt
Additional Tryptase alpha/beta-1 Products
Product Documents for Human Tryptase alpha/beta-1 Antibody
Product Specific Notices for Human Tryptase alpha/beta-1 Antibody
For research use only
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