Human VHL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB114351
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1066409
Product Specifications
Immunogen
E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337
Asn150-Asp213
Accession # P40337
Specificity
Detects human VHL in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VHL Antibody
Detection of VHL in Human Kidney.
VHL was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB114351) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human VHL Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney
Sample: Immersion fixed paraffin-embedded sections of Human Kidney
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VHL
References
- Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
- Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
- Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
- Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411
Long Name
Von Hippel-Lindau Tumor Suppressor
Alternate Names
Protein G7, pVHL
Gene Symbol
VHL
UniProt
Additional VHL Products
Product Documents for Human VHL Antibody
Product Specific Notices for Human VHL Antibody
For research use only
Loading...
Loading...
Loading...
Loading...