Human XCR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF857
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Specificity
Detects human XCR1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human XCR1 Antibody
Detection of XCR1 in Human Monocytes by Flow Cytometry.
Human peripheral blood monocytes were treated for 16 hours with 1 µg/mL LPS then stained with Goat Anti-Human XCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF857, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Detection of XCR1 in NS0 Mouse Cell Line Transfected with Human XCR-1 and eGFP by Flow Cytometry.
NS0 mouse myeloma cell line transfected with human XCR-1 and eGFP was stained with and either (A) Goat Anti-Human XCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF857) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.Applications for Human XCR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood monocytes treated with LPS and NS0 mouse myeloma cell line transfected with human XCR-1 and eGFP
Sample: Human peripheral blood monocytes treated with LPS and NS0 mouse myeloma cell line transfected with human XCR-1 and eGFP
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Sample: Immersion fixed human peripheral blood mononuclear cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human XCR1
Sample: Recombinant Human XCR1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCR1
XCR1, also known as GPR5 and lymphotactin/SCM-1 (single cysteine motif 1) receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, human herpesvirus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
Long Name
Chemokine [C Motif] Receptor 1
Alternate Names
CCXCR1, GPR5
Gene Symbol
XCR1
UniProt
Additional XCR1 Products
Product Documents for Human XCR1 Antibody
Product Specific Notices for Human XCR1 Antibody
For research use only
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