Human XCR1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF857
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-Val190, Phe251-Tyr267
Accession # P46094
Met1-Thr31, Ser89-Lys103, His168-Val190, Phe251-Tyr267
Accession # P46094
Specificity
Detects human XCR1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human XCR1 Biotinylated Antibody
Detection of XCR1 in Human LPS-treated PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells were treated for 16 hours with 1 µg/mL LPS then stained with Goat Anti-Human XCR1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF857, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).Applications for Human XCR1 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with LPS
Sample: Human peripheral blood mononuclear cells treated with LPS
Western Blot
0.1 µg/mL
Sample: Recombinant Human XCR1
Sample: Recombinant Human XCR1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCR1
XCR1, also known as GPR5 and lymphotactin/SCM-1 (single cysteine motif 1) receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, human herpesvirus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.
Long Name
Chemokine [C Motif] Receptor 1
Alternate Names
CCXCR1, GPR5
Gene Symbol
XCR1
UniProt
Additional XCR1 Products
Product Documents for Human XCR1 Biotinylated Antibody
Product Specific Notices for Human XCR1 Biotinylated Antibody
For research use only
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