This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Spectra Viewer
Flow Cytometry

ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58732PECY7

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all ICAM-1/CD54 Antibodies »

Product Summary for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Immunogen

Specificity

Clonality

Host

Isotype

Applications for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: ICAM-1/CD54

Long Name

Alternate Names

Gene Symbol

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ICAM-1/CD54 Antibody (1H4) [PE/Cy7]

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