ICAM-1/CD54 Antibody (1H4) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-582

Novus Biologicals, part of Bio-Techne
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NB500-582-100Tests
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Raji cells and spleen cells fused with NS1 cells.

Specificity

This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-1/CD54 Antibody (1H4) [PE]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.

Applications for ICAM-1/CD54 Antibody (1H4) [PE]

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ICAM-1/CD54

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1H4) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ICAM-1/CD54 Antibody (1H4) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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