ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-58732
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0007.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
![Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cell-Surface-NB110-58732-img0010.jpg "Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1H4
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Immunogen
Raji cells and spleen cells fused with NS1 cells.
Specificity
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]
Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0004.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0005.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Fig. 1. Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis. Image using the Biotin form of this antibody.![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0009.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]](https://resources.bio-techne.com/images/products/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0006.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]")
Applications for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
5-10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry-Paraffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required.
Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.
This antibody is CyTOF ready.
Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
(PBS), approx. pH 7.4; 0.2 um filter sterilized.
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Product Specific Notices for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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