IgG1 Antibody (RM117)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62028
Recombinant Monoclonal Antibody.
![ELISA: IgG1 Antibody (RM117) [NBP2-62028]](https://resources.bio-techne.com/images/products/IgG1-Antibody-RM117-ELISA-NBP2-62028-img0010.jpg "ELISA: IgG1 Antibody (RM117) [NBP2-62028]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Capture, Sandwich ELISA Detection
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM117
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide corresponding to the hinge region of Human IgG1
Specificity
This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG1 Antibody (RM117)
ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA of human immunoglobulins shows NBP2-62028 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins.NBP2-62028 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - Sandwich ELISA using NBP2-62028 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody NBP2-77396 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA: IgG1 Antibody (RM117) [Unconjugated] [NBP2-62028] - ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG bindingantibody (positive control) was used as the detection antibody.
Applications for IgG1 Antibody (RM117)
Application
Recommended Usage
Flow Cytometry
2ug/ml-8ug/ml
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Immunohistochemistry-Paraffin
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG1
Long Name
Immunoglobulin G1
Alternate Names
IgG1, Igh-4, RGD1359539, VH7183
Additional IgG1 Products
Product Documents for IgG1 Antibody (RM117)
Product Specific Notices for IgG1 Antibody (RM117)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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