IgG1 Antibody (RM117) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62028B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM117
Concentration
1 mg/ml
Product Summary for IgG1 Antibody (RM117) [Biotin]
Immunogen
Peptide corresponding to the hinge region of Human IgG1
Specificity
This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG1 Antibody (RM117) [Biotin]
ELISA: IgG1 Antibody (RM117) [Biotin] [NBP2-62028B] - ELISA of human immunoglobulins shows NBP2-62028B only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins. NBP2-62028B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG1 Antibody (RM117) [Biotin] [NBP2-62028B] - Sandwich ELISA using NBP2-62028B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
ELISA: IgG1 Antibody (RM117) [Biotin] [NBP2-62028B] - Sandwich ELISA using NBP2-62028B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
Applications for IgG1 Antibody (RM117) [Biotin]
Application
Recommended Usage
Flow Cytometry
2ug/ml-8ug/ml
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG1
Long Name
Immunoglobulin G1
Alternate Names
IgG1, Igh-4, RGD1359539, VH7183
Additional IgG1 Products
Product Documents for IgG1 Antibody (RM117) [Biotin]
Product Specific Notices for IgG1 Antibody (RM117) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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