IgG1 Antibody (RM117) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25974
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM117
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide corresponding to the hinge region of Human IgG1
Specificity
This antibody reacts to the heavy chain of human IgG1. It does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG1 Antibody (RM117) - Azide and BSA Free
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] -
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] - ELISA of human immunoglobulins shows RM117 only reacts to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] -
Sandwich ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] - Sandwich ELISA using RM117 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] -
ELISA: IgG1 Antibody (RM117) - Azide and BSA Free [NBP3-25974] - A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG1 Antibody (RM117) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2ug/ml-8ug/ml
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Immunohistochemistry-Paraffin
0.5ug/ml - 2ug/ml
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG1
Long Name
Immunoglobulin G1
Alternate Names
IgG1, Igh-4, RGD1359539, VH7183
Additional IgG1 Products
Product Documents for IgG1 Antibody (RM117) - Azide and BSA Free
Product Specific Notices for IgG1 Antibody (RM117) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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