IkB-alpha Antibody (116)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90043

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 116

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human IkB-alpha (Phe2-Leu317; Accession#: NP_065390.1)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for IkB-alpha Antibody (116)

Western Blot: IkB-alpha Antibody (116) [NBP2-90043]

Western Blot: IkB-alpha Antibody (116) [NBP2-90043]

Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa
Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043]

Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043]

Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg Hela Whole Cell LysateLane E:0.5 mg K562 Whole Cell Lysate2 uL anti-NFKBIA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa

Applications for IkB-alpha Antibody (116)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody (116)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IkB-alpha Antibody (116)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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