IkB-alpha Antibody (4D4C4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52517

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-52517
NBP2-52517-0.025mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D4C4

Format

BSA Free

Concentration

1 mg/ml

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human IkB-alpha (AA: 150-291) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IkB-alpha Antibody (4D4C4) - BSA Free

Western Blot: IkB-alpha Antibody (4D4C4)BSA Free [NBP2-52517]

Western Blot: IkB-alpha Antibody (4D4C4)BSA Free [NBP2-52517]

Western Blot: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)
Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (4D4C4) - BSA Free [NBP2-52517]

Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (4D4C4) - BSA Free [NBP2-52517]

Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of MCF-7 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) - BSA Free [NBP2-52517]

Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) - BSA Free [NBP2-52517]

Immunohistochemistry-Paraffin: IkB-alpha Antibody (4D4C4) [NBP2-52517] - Analysis of paraffin-embedded brain tissues using NFKBIA mouse mAb with DAB staining.
View 5 more images

Applications for IkB-alpha Antibody (4D4C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody (4D4C4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IkB-alpha Antibody (4D4C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.