IkB-alpha [p Ser36] Antibody (JE56-65)

Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773]

Western Blot: IkB-alpha [p Ser36] Antibody (JE56-65) [NBP3-32773] - Western blot analysis of IkB-alpha on different lysates with Rabbit anti-IkB-alpha antibody (NBP3-32773) at 1/1,000 dilution.

Lane 1: C6 cell lysate
Lane 2: C6 treated with 100n/mL Calyculin A for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 36 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes 45 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32773) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.