IkB-alpha [p Ser36] Antibody (SR1657)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21594

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21594-100ul
NBP3-21594-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1657

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-IkB-alpha [Ser36] (Uniprot #: P25963)

Modification

p Ser 36

Specificity

Detects endogenous levels of total phospho-IkB-alpha [Ser36]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IkB-alpha [p Ser36] Antibody (SR1657)

Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] -

Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.

Applications for IkB-alpha [p Ser36] Antibody (SR1657)

Application
Recommended Usage

Flow Cytometry

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser36] Antibody (SR1657)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IkB-alpha [p Ser36] Antibody (SR1657)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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