IkB-alpha [p Ser32, p Ser36] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81987

Novus Biologicals, part of Bio-Techne
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NB100-81987
NB100-81987-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human IkB-alpha. around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).

Modification

p Ser32, p Ser36

Specificity

Detects endogenous levels of IkB-alpha only when phosphorylated at serine 32/36.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IkB-alpha [p Ser32, p Ser36] Antibody

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] -

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of extracts from Hela cells untreated or treated with TNF using NB100-81987

Immunocytochemistry/Immunofluorescence: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] -

Immunocytochemistry/Immunofluorescence: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Staining of methanol-fixed Hela cells using NB100-81987

Immunohistochemistry-Paraffin: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] -

Immunohistochemistry-Paraffin: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81987 (left) or the same antibody preincubated with blocking peptide (right).

Applications for IkB-alpha [p Ser32, p Ser36] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Entrez Gene IDs

4792 (Human); 18035 (Mouse); 25493 (Rat)

Gene Symbol

NFKBIA

UniProt

P25963

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser32, p Ser36] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IkB-alpha [p Ser32, p Ser36] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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