IkB-alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23384

Novus Biologicals, part of Bio-Techne
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NBP3-23384
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

IkB-alpha (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IkB-alpha. This peptide sequence has high homology to rat and mouse IkB-alpha, and low homology to other IkB proteins. [UniProt# P25963]

Specificity

This antibody detects a 38 kDa protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for IkB-alpha Antibody

IkB-alpha Antibody

Western Blot: IkB-alpha Antibody [NBP3-23384] -

Western Blot: IkB-alpha Antibody [NBP3-23384] - Western blot image of human A431. The Blots were probed with anti-IkB-alpha (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).

Applications for IkB-alpha Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IkB-alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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