MAP3K8/Tpl2/COT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86569

Novus Biologicals, part of Bio-Techne
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NBP1-86569
NBP1-86569-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP3K8/Tpl2/COT Antibodies »

Product Summary for MAP3K8/Tpl2/COT Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K8/Tpl2/COT Antibody

Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Analysis in control (vector only transfected HEK293T lysate) and MAP3K8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Immunocytochemistry/ Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Immunocytochemistry/Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-86569]

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

Applications for MAP3K8/Tpl2/COT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP3K8/Tpl2/COT

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Alternate Names

COT, EST, ESTF, MAP3K8, Tpl-2

Gene Symbol

MAP3K8

Additional MAP3K8/Tpl2/COT Products

Product Documents for MAP3K8/Tpl2/COT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAP3K8/Tpl2/COT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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