Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72598
![Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Western-Blot-NBP2-72598-img0001.jpg "Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]")
![Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Western-Blot-NBP2-72598-img0002.jpg "Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI10F6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
Western Blot: Mcl-1 Antibody (OTI10F6)Azide and BSA Free [NBP2-72598]
Western Blot: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL-1.![Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72598-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]")
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]
Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MCL-1.![Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]](https://resources.bio-techne.com/images/products/Mcl-1-Antibody-OTI10F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72598-img0003.jpg "Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]")
Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598]
Flow Cytometry: Mcl-1 Antibody (OTI10F6) - Azide and BSA Free [NBP2-72598] - HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal and then analyzed by flow cytometry.Applications for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:200 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Mcl-1
Long Name
Myeloid Cell Leukemia Sequence 1
Alternate Names
BCL2L3, Mcl1
Gene Symbol
MCL1
Additional Mcl-1 Products
Product Documents for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
Product Specific Notices for Mcl-1 Antibody (OTI10F6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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