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Mouse CD96 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5690R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 630612

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD96
Val22-Met536
Accession # Q3U0X8

Specificity

Detects mouse CD96 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD96v2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CD96 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily.  It is expressed on CD4+ and CD8+ T cells, NK and NKT cells, resting monocytes and gamma delta T cells.  Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion.  Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein.  It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region.  The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355).  Unlike human, there is no splice variant in the second V-type domain.  There is, however, a potential isoform that shows a single Cys substitution for aa 437-602.  Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Additional CD96 Products

Product Documents for Mouse CD96 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD96 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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