Mouse splenocytes were stained with Rat Anti-Mouse CD96 PE-conjugated Monoclonal Antibody (Catalog # FAB5690P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD96 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma delta T cells. Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355). Unlike human, there is no splice variant in the second V-type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437-602. Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Q3U0X8

Additional CD96 Products

Product Documents for Mouse CD96 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD96 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD96 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5690P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD96 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD96 PE-conjugated Antibody

Detection of CD96 in Mouse Splenocytes by Flow Cytometry.