Mouse splenocytes were labeled with Mouse CD96 Monoclonal Antibody (Catalog # MAB5690) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113) and Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Applications for Mouse CD96 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma delta T cells. Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion. Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein. It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region. The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355). Unlike human, there is no splice variant in the second V-type domain. There is, however, a potential isoform that shows a single Cys substitution for aa 437-602. Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Q3U0X8

Additional CD96 Products

Product Documents for Mouse CD96 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD96 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD96 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5690

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all CD96 Antibodies »

Product Summary for Mouse CD96 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD96 Antibody

Detection of CD96 in Mouse Splenocytes by Flow Cytometry.