Mouse IgG2B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9636
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2215A
Product Summary for Mouse IgG2B Antibody
Immunogen
Mouse IgG
Specificity
Detects mouse IgG2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG1, mouse IgG2A, and mouse IgG3 was observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse IgG2B Antibody
Detection of Mouse IgG2BPrimary Antibodies by Western Blot.
Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (MAB3286, Mouse IgG2Bisotype), anti-HSP60 (MAB1800, Mouse IgG2Bisotype), anti-GAPDH (MAB5718,Mouse IgG1isotype), anti-Peroxiredoxin 6 (MAB3490, Mouse IgG3isotype), and anti-SOD1 (MAB3418, Mouse IgG2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2B(Catalog # MAB9636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for IgG2Bisotype antibodies at approximately 60 and 90 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) but not Rabbit Anti-Mouse IgG2BMonoclonal Antibody (Catalog # MAB9636). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IgG2BPrimary Antibody by Anti-Mouse IgG2B-specific Secondary Antibody.
Human PBMC lymphocytes were stained with Mouse anti-Human CD3e Monoclonal Antibody (MAB100, Mouse IgG1isotype), Mouse anti-Human CD4 Monoclonal Antibody (MAB3791, Mouse IgG2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (MAB1509, Mouse IgG2Bisotype), followed by Rabbit anti-Mouse IgG2B-specific secondary antibody (Catalog # MAB9636) then PE-conjugated Goat anti-Rabbit secondary antibody (F0110). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse IgG2BPrimary Antibody by Anti-Mouse IgG2B-specific Secondary Antibody.
Human PBMC lymphocytes were stained with Mouse anti-Human DTK Monoclonal Antibody (Catalog # MAB859, Mouse IgG1 isotype; aqua histogram), Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB13001, Mouse IgG2A isotype; dark blue histogram), or Mouse anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, Mouse IgG2B isotype; orange histogram), followed by Rabbit anti-Mouse IgG2B-specific secondary antibody (Catalog # MAB9636) then APC-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse IgG2B Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Designed as a secondary antibody to detect cells stained with a primary antibody of mouse IgG2B isotype
Sample: Designed as a secondary antibody to detect cells stained with a primary antibody of mouse IgG2B isotype
Western Blot
0.5 µg/mL
Sample: Mouse IgG2B Isotype Primary Antibodies
Sample: Mouse IgG2B Isotype Primary Antibodies
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2B Fc
Additional IgG2B Products
Product Documents for Mouse IgG2B Antibody
Product Specific Notices for Mouse IgG2B Antibody
For research use only
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